Regulatory

Part:BBa_K2605001:Design

Designed by: Kaitlin Schaaf   Group: iGEM18_Calgary   (2018-10-09)


CMV with 5' SalI restriction site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A SalI restriction site (GTCGAC) was added to the 5' end of the original promoter sequence via overhang PCR. This restriction site makes the promoter compatible with our 

 Multiple-Cloning Site flanked by FRT sites Part BBa_K2605002 system.

Source

BBa_K747096

References